Kenji YOKOYAMA
| Name | Kenji YOKOYAMA |
| Department/Research Field | School of Bionics/Applied biochemistry and healthcare technology |
| Research content |
| Our laboratory works on applied biochemistry and healthcare technology. For instance, continuous blood glucose monitoring system is developed based on enzyme catalyzed electrochemical devices with redox active polymer. Ferrocene-containing redox active polymer is immobilized on an electrode and used as electron transfer mediator for continuous glucose monitoring. Enzyme-based biomass conversion and synthesis of fine chemicals and valuable food stuff are investigated. Ionic liquids are employed for enzyme reaction, for instance, cellulose hydrolysis using cellulase. Hydrolysis reaction is accelerated in aqueous solution containing ionic liquid. Oligosaccharide can be synthesized using reverse reaction of hydrolysis enzyme in aqueous solution containing ionic liquid. Highly sensitive analysis for disease and health biomarkers and valuable food substances is investigated based on HPLC electrochemical detection. Biomarkers, such as oxidative stress marker 8-hydroxy-2’-deoxyguanosine (8-OHdG), and spice substances, such as capsaicin derivatives, are analyzed. Reverse phase liquid chromatography is employed and combined with electrochemical detector with two working electrodes at two different applied potentials. Electrochemical sensor devices for such target molecules are also investigated. Highly sensitive electrochemical detection can be achieved using electron transfer mediators. |
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| Research theme |
1. Continuous blood glucose monitoring |
| Research keywords |
| clinical analysis , enzyme-based production , electrochemical HPLC analysis |
| Lab link |